dual luciferase reporter assay kit Search Results


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Vazyme Biotech Co luciferase activity
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MedChemExpress renilla firefly luciferase dual assay kit mce cat
Renilla Firefly Luciferase Dual Assay Kit Mce Cat, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega dual luciferase kit
Dual Luciferase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega dual luciferase reporter assay kit
Dual Luciferase Reporter Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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INDIGO Biosciences cell-based human rar-driven luciferase reporter assay
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Beyotime dual-luciferase activity assays rg027
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Beijing Solarbio Science dual luciferase assay kit d0010
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Yeasen Biotechnology dual luciferase reporter assay kit
The MALAT1/miR-92a-3p axis regulates ADAM10 expression. (A) IMR-32 cells were treated with 2 μM MLN8237, 0.5 μM DOX, DMSO, or no treatment as a control. (B) After 72 h, miR-92a-3p and ADAM10 expression was detected by qRT-PCR. (C) Prediction of binding sites between miR-92a-3p and MALAT1 and those between miR-92a-3p and ADAM10 using ENCORI. (D) Confirmation of miR-92a-3p binding to ADMA10 and MALAT1 was confirmed by <t>luciferase-reporter</t> assay using 293T cells. (E) IMR-32 was co-transfected with an miR-92a-3p inhibitor and si-MALAT1, and ADAM10 level was detected by western blot. (F) The expression of miR-92a-3p and ADAM10 was detected by qRT-PCR. Data represent the mean ± SEM of three independent experiments. * p < 0.05.
Dual Luciferase Reporter Assay Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ribobio co dual-luciferase reporter assay kit
The MALAT1/miR-92a-3p axis regulates ADAM10 expression. (A) IMR-32 cells were treated with 2 μM MLN8237, 0.5 μM DOX, DMSO, or no treatment as a control. (B) After 72 h, miR-92a-3p and ADAM10 expression was detected by qRT-PCR. (C) Prediction of binding sites between miR-92a-3p and MALAT1 and those between miR-92a-3p and ADAM10 using ENCORI. (D) Confirmation of miR-92a-3p binding to ADMA10 and MALAT1 was confirmed by <t>luciferase-reporter</t> assay using 293T cells. (E) IMR-32 was co-transfected with an miR-92a-3p inhibitor and si-MALAT1, and ADAM10 level was detected by western blot. (F) The expression of miR-92a-3p and ADAM10 was detected by qRT-PCR. Data represent the mean ± SEM of three independent experiments. * p < 0.05.
Dual Luciferase Reporter Assay Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TransGen biotech co double-luciferase reporter assay kit
The MALAT1/miR-92a-3p axis regulates ADAM10 expression. (A) IMR-32 cells were treated with 2 μM MLN8237, 0.5 μM DOX, DMSO, or no treatment as a control. (B) After 72 h, miR-92a-3p and ADAM10 expression was detected by qRT-PCR. (C) Prediction of binding sites between miR-92a-3p and MALAT1 and those between miR-92a-3p and ADAM10 using ENCORI. (D) Confirmation of miR-92a-3p binding to ADMA10 and MALAT1 was confirmed by <t>luciferase-reporter</t> assay using 293T cells. (E) IMR-32 was co-transfected with an miR-92a-3p inhibitor and si-MALAT1, and ADAM10 level was detected by western blot. (F) The expression of miR-92a-3p and ADAM10 was detected by qRT-PCR. Data represent the mean ± SEM of three independent experiments. * p < 0.05.
Double Luciferase Reporter Assay Kit, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Packard Instruments luclite luciferase reporter gene assay kit
The MALAT1/miR-92a-3p axis regulates ADAM10 expression. (A) IMR-32 cells were treated with 2 μM MLN8237, 0.5 μM DOX, DMSO, or no treatment as a control. (B) After 72 h, miR-92a-3p and ADAM10 expression was detected by qRT-PCR. (C) Prediction of binding sites between miR-92a-3p and MALAT1 and those between miR-92a-3p and ADAM10 using ENCORI. (D) Confirmation of miR-92a-3p binding to ADMA10 and MALAT1 was confirmed by <t>luciferase-reporter</t> assay using 293T cells. (E) IMR-32 was co-transfected with an miR-92a-3p inhibitor and si-MALAT1, and ADAM10 level was detected by western blot. (F) The expression of miR-92a-3p and ADAM10 was detected by qRT-PCR. Data represent the mean ± SEM of three independent experiments. * p < 0.05.
Luclite Luciferase Reporter Gene Assay Kit, supplied by Packard Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Melone Pharmaceutical Co Ltd firefly luciferase reporter assay kit
The MALAT1/miR-92a-3p axis regulates ADAM10 expression. (A) IMR-32 cells were treated with 2 μM MLN8237, 0.5 μM DOX, DMSO, or no treatment as a control. (B) After 72 h, miR-92a-3p and ADAM10 expression was detected by qRT-PCR. (C) Prediction of binding sites between miR-92a-3p and MALAT1 and those between miR-92a-3p and ADAM10 using ENCORI. (D) Confirmation of miR-92a-3p binding to ADMA10 and MALAT1 was confirmed by <t>luciferase-reporter</t> assay using 293T cells. (E) IMR-32 was co-transfected with an miR-92a-3p inhibitor and si-MALAT1, and ADAM10 level was detected by western blot. (F) The expression of miR-92a-3p and ADAM10 was detected by qRT-PCR. Data represent the mean ± SEM of three independent experiments. * p < 0.05.
Firefly Luciferase Reporter Assay Kit, supplied by Melone Pharmaceutical Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The MALAT1/miR-92a-3p axis regulates ADAM10 expression. (A) IMR-32 cells were treated with 2 μM MLN8237, 0.5 μM DOX, DMSO, or no treatment as a control. (B) After 72 h, miR-92a-3p and ADAM10 expression was detected by qRT-PCR. (C) Prediction of binding sites between miR-92a-3p and MALAT1 and those between miR-92a-3p and ADAM10 using ENCORI. (D) Confirmation of miR-92a-3p binding to ADMA10 and MALAT1 was confirmed by luciferase-reporter assay using 293T cells. (E) IMR-32 was co-transfected with an miR-92a-3p inhibitor and si-MALAT1, and ADAM10 level was detected by western blot. (F) The expression of miR-92a-3p and ADAM10 was detected by qRT-PCR. Data represent the mean ± SEM of three independent experiments. * p < 0.05.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Mechanisms of Senescence-Related NKG2D Ligands Release and Immune Escape Induced by Chemotherapy in Neuroblastoma Cells

doi: 10.3389/fcell.2022.829404

Figure Lengend Snippet: The MALAT1/miR-92a-3p axis regulates ADAM10 expression. (A) IMR-32 cells were treated with 2 μM MLN8237, 0.5 μM DOX, DMSO, or no treatment as a control. (B) After 72 h, miR-92a-3p and ADAM10 expression was detected by qRT-PCR. (C) Prediction of binding sites between miR-92a-3p and MALAT1 and those between miR-92a-3p and ADAM10 using ENCORI. (D) Confirmation of miR-92a-3p binding to ADMA10 and MALAT1 was confirmed by luciferase-reporter assay using 293T cells. (E) IMR-32 was co-transfected with an miR-92a-3p inhibitor and si-MALAT1, and ADAM10 level was detected by western blot. (F) The expression of miR-92a-3p and ADAM10 was detected by qRT-PCR. Data represent the mean ± SEM of three independent experiments. * p < 0.05.

Article Snippet: Luciferase activity was detected using the Dual Luciferase Reporter Assay kit (YEASEN) after 48 h, Renilla luciferase activity was normalized to Firefly luciferase activity for each transfected well.

Techniques: Expressing, Control, Quantitative RT-PCR, Binding Assay, Luciferase, Reporter Assay, Transfection, Western Blot