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Image Search Results
Journal: Frontiers in Cell and Developmental Biology
Article Title: Mechanisms of Senescence-Related NKG2D Ligands Release and Immune Escape Induced by Chemotherapy in Neuroblastoma Cells
doi: 10.3389/fcell.2022.829404
Figure Lengend Snippet: The MALAT1/miR-92a-3p axis regulates ADAM10 expression. (A) IMR-32 cells were treated with 2 μM MLN8237, 0.5 μM DOX, DMSO, or no treatment as a control. (B) After 72 h, miR-92a-3p and ADAM10 expression was detected by qRT-PCR. (C) Prediction of binding sites between miR-92a-3p and MALAT1 and those between miR-92a-3p and ADAM10 using ENCORI. (D) Confirmation of miR-92a-3p binding to ADMA10 and MALAT1 was confirmed by luciferase-reporter assay using 293T cells. (E) IMR-32 was co-transfected with an miR-92a-3p inhibitor and si-MALAT1, and ADAM10 level was detected by western blot. (F) The expression of miR-92a-3p and ADAM10 was detected by qRT-PCR. Data represent the mean ± SEM of three independent experiments. * p < 0.05.
Article Snippet: Luciferase activity was detected using the
Techniques: Expressing, Control, Quantitative RT-PCR, Binding Assay, Luciferase, Reporter Assay, Transfection, Western Blot